Cell responses to surface and contact cell guidance are of great interest in bio-applications especially on nano- and micro scale features. Recently we showed selective cell responses on Al/Al2O3bi-phasic nanowires (NWs). In this context, Al/Al2O3NWs were synthesized by the chemical vapor deposition of (tBuOAlH2)2. Afterwards, linear periodic nano- and micro structured NWs were formed using laser interference lithography (LIL) technique to study the contact guidance of neurons from rat dorsal root ganglion (DRG), human umbilical vein smooth muscle cells (HUVSMC), human umbilical vein endothelial cells (HUVEC) and human osteoblast (HOB). LIL treatment did not alter surface chemistry of NWs. From our preliminary research LIL patterned NWs lead to alignment of axons contrary to non-patterned NWs. Morphology of HUVSMC changed from poly- to linear shapes and strong alignment was observed while HUVEC and HOB were not affected.