Materials-Host Interactions

To understand specific interactions between LTMs (or other therapeutics) and their target tissue in the host, we have established several in vitro models, and we continue to develop new ones to incorporate to our portfolio. We focus on angiogenesis, inflammation and biological barriers like the cornea.

Investigation of angiogenic potential

We have developed 2D and 3D models to assess pro- and anti-angiogenic potential using primary human endothelial cells and dermal fibroblasts. We also use the chick chorioallantoic membrane assay, an ex vivo system for the assessment of new capillary formation. These models allow us to quantify several parameters during endothelial cell sprouting.

Models of endotoxemia

We are developing methods to assess inflammatory potential in vitro, by using cells involved in the foreign body reaction such as monocytes, macrophages and fibroblasts. We characterize phenotypic and morphological changes of these cells when in contact with LTMs or treated with different therapeutics. We use the monocyte activation test to detect the presence of endotoxin or non-endotoxin pyrogens, which can trigger fever in the host. We have developed an in vitro model of endotoxemia using macrophages, which can be used to investigate the mechanism of action of some therapeutics

Biological barriers models – Cornea

We are developing different barrier models, with a focus on the cornea tissue. We use these models to investigate drug-delivery to the eye. The complexity of biological barriers (skin, gut, blood-brain barrier, eye) comes from their layered structure with different cells that are critical for the overall function of the tissue. Many biological barriers are in contact with the air and therefore complex 3D layered models at the air-liquid interface are leveraged to recapitulate these tissues. These models will help us understand for example, how LTMs can be applied to the eye.