Materials-Host Interactions

To understand specific interactions between LTMs and their target tissue in the host, we have established in vitro models, and we continue to develop new ones to incorporate to our portfolio. We focus on angiogenesis, inflammation and the cornea.

Angiogenesis

We have developed 2D and 3D models to assess pro- and anti-angiogenic potential using endothelial cells and dermal fibroblasts. We also perform the chick chorioallantoic membrane assay, an ex vivo system for the quantification of angiogenesis. These models allow us to quantify several parameters during endothelial cell sprouting to characterize the potential of a treatment to trigger capillary formation.

Inflammation

We are developing methods to assess inflammatory potential in vitro, by using cells involved in the foreign body reaction to biomaterials such as monocytes, macrophages and fibroblasts. We look for ways to detect changes on these cells when in contact with LTMs. We use the monocyte activation test to detect the presence of endotoxin or non-endotoxin pyrogens, which can trigger fever in the host. We characterise the response of macrophages to LTMs and study the effects of anti-inflammatory LTMs.

In vitro cornea

We are developing models of the cornea tissue to investigate drug-delivery to the eye. These models will help us understand for example, how LTMs can be applied to the eye.